Brad Day, Assistant Professor
Contact information:
517-353-7991
bday@msu.edu
Day Lab Website
Education:
B.S., The University of Tennessee, Microbiology
Ph.D., The University of Tennessee, Microbiology
General areas of expertise:
Molecular Biology of Host-Pathogen Interactions
Overview of current program:
Research in my laboratory focuses on the molecular-genetics of innate immunity in plants. Using a combination of molecular biology and biochemical approaches, we are interested in understanding the signaling mechanisms which lead to the activation of host defense responses following pathogen perception. Understanding host specificity, as well as the specificity which regulates the activation of these responses is key to understanding plant disease resistance. We are currently using the model interaction, Arabidopsis thaliana-Pseudomonas syringae, to address questions related to 1) the involvement of the actin cytoskeleton, and its association with gene-for-gene resistance responses, 2) the characterization of a novel GPI-anchored protein required for defense signaling, and 3) the identification of conserved signaling mechanisms which contribute to the activation of defenses in a broad range of plant-pathogen interactions. For the latter, we are currently conducting several mutagenesis screens to identify genetic loci which contribute to resistance in Arabidopsis not to only P. syringae, but also Colletotrichum spp., one of the most important and common fungal genus causing anthracnose or more than 50 host plant species.
Publications (most recent shown):
Day, B., Dahlbeck, D., and Staskawicz, B.J. (2006). The RIN4-NDR1 interaction mediates the differential activation of at least three members of the CC-NB-LRR class of resistance proteins in Arabidopsis thaliana. Plant Cell. 18: 1-10.
Tian, M., and Day, B. (2006). Domain switching and host recognition. Mol. Micro. 61: 1091-1093.
Chisholm, S*., Coaker, G*., Day, B*., and Staskawicz, B.J. (2006). Host-Microbe Interactions: Shaping the Evolution of the Plant Immune Response. Cell. 124: 1-12. *Co-First Authors.
Day, B., Dahlbeck, D., Huang, J., Chisholm, S.T., Li, D., and Staskawicz, B.J. (2005). Molecular basis for the RIN4 negative regulation of RPS2 disease resistance. Plant Cell. 17: 1292–1305.
Chisholm ST, Dahlbeck D, Krishnamurthy N, Day B, Sjolander K, Staskawicz BJ. (2005). Molecular characterization of proteolytic cleavage sites of the Pseudomonas syringae effector AvrRpt2. Proc. Natl. Acad. Sci. USA. 102:2087-2092.
Leister, R.T., Dahlbeck, D., Day, B., Li, Y., Chesnokova, O., and Staskawicz, B.J. 2005. Molecular Genetic Evidence for the Role of NbSGT1 in the Intra-Molecular Complementation of Bs2 Protein Activity in Nicotiana benthamiana. Plant Cell. 17: 1268-1278.
Coppinger, J.P, Repetti, P., Day, B, Dahlbeck, D., Mehlert, A., and Staskawicz, B. 2004. Overexpression of the plasma membrane-localized NDR1 protein results in enhanced bacterial disease resistance in Arabidopsis thaliana. Plant J. 40: 225-237.
R. Bradley Day, Masaji Koshioka, Toshiaki Mitsui, Miyako Ueguchi-Tanaka, Makoto Matsuoka, Naoto Shibuya and Eiichi Minami. 2004. Expression of Two Members of the Rice Family of GRAS Genes, CIGR1 and CIGR2, are Induced by Gibberellin: Evidence for Cross Talk between Chitin and GA Signaling. Plant Mol. Biol. 54:261-272.
R. Bradley Day, Naoto Shibuya and Eiichi Minami. 2003. DNA Microarray Analysis of Chitin-Treated Rice Cells: Identification and Characterization of Two New Members of the GRAS Gene Family. Biochim. Biophys. Acta. 1625:261-268.
Robert B. Day, Chiharu Akimoto, Yoko Nishizawa, Junshi Yazaki, Keiko Nakamura, Fumiko Fujii, Kanako Shimbo, Kimiko Yamamoto, Katsumi Sakata, Takuji Sasaki, Naoki Kishimoto, Shoshi Kikuchi, Naoto Shibuya and Eiichi Minami. 2002. Large-scale identification of elicitor-responsive genes in suspension-cultured rice cells by DNA microarray. Plant Biotechnology. 19: 153-155.
Day, R. B., Okada, M., Ito, Y., Tsukada, K., Zaghouani, H., Shibuya, N, and Stacey, G. 2000. A High-Affinity Binding Site for Chitin Oligosaccharides is localized in the Plasma Membrane of Soybean. Plant Physiol. 126: 1162-1173.
R. Bradley Day, Crystal McAlvin, John Loh, Roxanne Denny, John Dunlap, Nevin Young, and Gary Stacey. 2000. Differential Expression of Two Soybean Apyrases; one of which is an early nodulin. Mol. Plant-Microbe Interact. 13: 1053-1070.
Courses taught:
PLP 881, Biochemical and Molecular Plant Pathology